Dissection of affinity captured LINE-1 macromolecular complexes
نویسندگان
چکیده
Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.
منابع مشابه
Dissection of affinity captured LINE - 1 macromolecular 1 complexes
Massachusetts General Hospital, Department of Pathology (Boston, MA, United States); Moscow 6 Institute of Physics and Technology, (Dolgoprudny, Russian Federation); The Rockefeller 7 University, Laboratory of Mass Spectrometry and Gaseous Ion Chemistry (New York, NY, United 8 States); Institute for Systems Genetics, NYU Langone Health, Department of Biochemistry and 9 Molecular Pharmacology (N...
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